| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.193441 |
| Chromosome: | chromosome 16 |
| Location: | 1480685 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g652850 | ALG5,GTR4 | Dolichyl-phosphate beta-glucosyltransferase; (1 of 1) 2.4.1.117 - Dolichyl-phosphate beta-glucosyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGTGAATGGCAGAGGACAAACAGTTTGGC |
| Internal bar code: | GACGTAAAGTGGCTCAGTATGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 305 |
| LEAP-Seq percent confirming: | 99.8162 |
| LEAP-Seq n confirming: | 1629 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCAGCATTTTTCTGACTGG |
| Suggested primer 2: | GGCGTTAACGTTGAACCAAT |