| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.193468 |
| Chromosome: | chromosome 16 |
| Location: | 1666860 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g654600 | PHC56 | (1 of 71) PF12499 - Pherophorin (DUF3707); Pherophorin-chlamydomonas homolog 56 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCCCCCTCCTCCCTCCCAACCTCCTCCCT |
| Internal bar code: | CGGTCTTTCGGTGGGCAAAATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 572 |
| LEAP-Seq percent confirming: | 94.7552 |
| LEAP-Seq n confirming: | 271 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCAGCTATCTTGCCCAAC |
| Suggested primer 2: | GATGGAGGAGGTGGAGAAGG |