| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.193490 |
| Chromosome: | chromosome 6 |
| Location: | 7401748 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g299500 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAAGGGGGGCAGGCTTAGTGCAGCGCGCT |
| Internal bar code: | CAGTACGACCCACGGGTACAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1086 |
| LEAP-Seq percent confirming: | 97.9646 |
| LEAP-Seq n confirming: | 2599 |
| LEAP-Seq n nonconfirming: | 54 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGACCCTGAGCTAGCCAAA |
| Suggested primer 2: | AACGTTCGGAGATGGATTTG |