| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.193533 |
| Chromosome: | chromosome 12 |
| Location: | 7664513 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g554350 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAGTGCCGGCCCTGAACGGCGGCAGCTGT |
| Internal bar code: | GCTCGGTGCCATTTGTGTGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 478 |
| LEAP-Seq percent confirming: | 99.6364 |
| LEAP-Seq n confirming: | 548 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGCAGATTGGAGCGGCTAT |
| Suggested primer 2: | CCTTACACGCACACATACCG |