| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.193544 |
| Chromosome: | chromosome 17 |
| Location: | 692443 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g700750 | GFY3 | (1 of 5) PTHR30178 - INNER MEMBRANE PROTEIN YAAH; Putative acetate transporter | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATTTACGCCATCCAGCCAGAAACCATACA |
| Internal bar code: | CGTAGCGTTGAGCGGGCCTACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 454 |
| LEAP-Seq percent confirming: | 93.186 |
| LEAP-Seq n confirming: | 506 |
| LEAP-Seq n nonconfirming: | 37 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGCCCTCAGAGACAGACC |
| Suggested primer 2: | GTGATGAGGTGACTGCGAGA |