| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.193596 |
| Chromosome: | chromosome 6 |
| Location: | 3979987 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278196 | (1 of 3) PF01575 - MaoC like domain (MaoC_dehydratas) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACCAAGTCTTTCCGCGGGGCTCTCTGTAC |
| Internal bar code: | CACAACTGCCTGAGCCGCCACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1 |
| LEAP-Seq percent confirming: | 99.72 |
| LEAP-Seq n confirming: | 3561 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTGATTAGGGAAGCTGTG |
| Suggested primer 2: | CACAACCAACACGAACAAGG |