Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.193664 |
Chromosome: | chromosome 14 |
Location: | 1476848 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre14.g618150 | (1 of 1) PF07058 - Microtubule-associated protein 70 (MAP70) | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGGATGCTGCTGGTGCTGGCGCGCACGG |
Internal bar code: | GGAACACCTTACGCTAGGTCAC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 523 |
LEAP-Seq percent confirming: | 99.4536 |
LEAP-Seq n confirming: | 182 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACTCAACTACGCAACCCCAC |
Suggested primer 2: | GCACCTTCAGCTTCTTACCG |