Insertion junction: LMJ.RY0402.193664_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.193664
Chromosome:chromosome 14
Location:1476848
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre14.g618150 (1 of 1) PF07058 - Microtubule-associated protein 70 (MAP70) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGGGATGCTGCTGGTGCTGGCGCGCACGG
Internal bar code:GGAACACCTTACGCTAGGTCAC

Confirmation - LEAP-Seq

LEAP-Seq distance:523
LEAP-Seq percent confirming:99.4536
LEAP-Seq n confirming:182
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:ACTCAACTACGCAACCCCAC
Suggested primer 2:GCACCTTCAGCTTCTTACCG