| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.193667 |
| Chromosome: | chromosome 10 |
| Location: | 3894852 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g447800 | (1 of 3) K11089 - 60 kDa SS-A/Ro ribonucleoprotein (TROVE2, SSA2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGCAGGCAAGGGTGGGGGCCAGGACGAAG |
| Internal bar code: | AGGGTTAGTAGCTTACACCGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1015 |
| LEAP-Seq percent confirming: | 99.6965 |
| LEAP-Seq n confirming: | 3613 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTAAGGCTGTCGCGTTCTC |
| Suggested primer 2: | TGGGCACGTACCATAGAACA |