Insertion junction: LMJ.RY0402.193708_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.193708
Chromosome:chromosome 2
Location:5033771
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g104450 (1 of 10) PTHR23257//PTHR23257:SF520 - SERINE-THREONINE PROTEIN KINASE // IP11267P intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGCTGCGTGCATGCGCCGCGGTAGATACG
Internal bar code:CACTTTTTCCTTCATACTCGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:574
LEAP-Seq percent confirming:93.219
LEAP-Seq n confirming:1141
LEAP-Seq n nonconfirming:83
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CCACAGAAATCGCAGTCTCA
Suggested primer 2:CCGTGTGTGTGTGTGTGTGT