| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.193720 |
| Chromosome: | chromosome 11 |
| Location: | 2385196 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g474700 | BES6 | (1 of 10) PF01062 - Bestrophin, RFP-TM, chloride channel (Bestrophin); putative chloride channel | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAACGCGGTCACGGATACTGCGTTTGGGCT |
| Internal bar code: | ACCCCGGGTATTCCTCGCCCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 945 |
| LEAP-Seq percent confirming: | 99.5452 |
| LEAP-Seq n confirming: | 1751 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGACGAGCGCATACATAA |
| Suggested primer 2: | GAAGGAAGGCTGAACTGTGC |