| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.193724 |
| Chromosome: | chromosome 16 |
| Location: | 4697983 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g687117 | (1 of 2) PF14249 - Tocopherol cyclase (Tocopherol_cycl) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCTATGCTCCATGATCCCACTTGCCCGCC |
| Internal bar code: | ATCGCGCCCAAGGGCTGCCCCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 500 |
| LEAP-Seq percent confirming: | 93.1153 |
| LEAP-Seq n confirming: | 541 |
| LEAP-Seq n nonconfirming: | 40 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAACAATCACCACACTGCCA |
| Suggested primer 2: | ACCAGTACTGCTGCACAACG |