| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.193730 |
| Chromosome: | chromosome 6 |
| Location: | 2300320 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g267100 | SRP68 | (1 of 1) K03107 - signal recognition particle subunit SRP68 (SRP68); Subunit of the Signal Recognition Particle | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTCCGCCTCGCTGCGTTCCCGTGTGCTG |
| Internal bar code: | TCCAGTACGCCCGCTGGGCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 941 |
| LEAP-Seq percent confirming: | 99.6858 |
| LEAP-Seq n confirming: | 3807 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTTGATGGTGTGTACGGC |
| Suggested primer 2: | ACTACACATTCGACCTGCCC |