| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.193778 |
| Chromosome: | chromosome 1 |
| Location: | 2302014 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g012600 | GPM2,PGM2 | Phosphoglucomutase 2; (1 of 1) 5.4.2.7 - Phosphopentomutase / Phosphodeoxyribomutase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCAGCGTACAGGGGCGGACCGCGTCGACA |
| Internal bar code: | GCCTCTGTGGGTCGATCCCTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 503 |
| LEAP-Seq percent confirming: | 97.5845 |
| LEAP-Seq n confirming: | 1010 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTTTTCATGGACGGCTGAT |
| Suggested primer 2: | CCGAGCGGTAGTCAAAGAAG |