Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.193782 |
Chromosome: | chromosome 7 |
Location: | 3520131 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre07.g336100 | (1 of 77) IPR029071 - Ubiquitin-related domain | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCTGCCGTTTACTTGCCCAACAAGAGGTG |
Internal bar code: | CTTGGAGCTTCTCAGGGGTGCA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 977 |
LEAP-Seq percent confirming: | 98.8922 |
LEAP-Seq n confirming: | 3035 |
LEAP-Seq n nonconfirming: | 34 |
LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACAAGCCCATGGCTGAATAC |
Suggested primer 2: | AACTAACAACGATCACCGGC |