| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.193796 |
| Chromosome: | chromosome 10 |
| Location: | 1697416 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g430250 | ELG21 | Exostosin-like glycosyltransferase 21; (1 of 2) IPR000742//IPR004263//IPR013111 - EGF-like domain // Exostosin-like // EGF-like domain, extracellular | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGTTCCGCTGGCTCGGTGTCAAGCCCCA |
| Internal bar code: | AAAAGGGTACGCTACGCAGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 420 |
| LEAP-Seq percent confirming: | 99.8621 |
| LEAP-Seq n confirming: | 1448 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGAAGCAAGACACGTTGAC |
| Suggested primer 2: | CGGGTTTAAAGCTCTCGATG |