| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.193797 |
| Chromosome: | chromosome 17 |
| Location: | 3187835 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g721950 | (1 of 1) PTHR11685:SF119 - E3 UBIQUITIN-PROTEIN LIGASE ARI2-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACGGACGCGGGGTCAGCGGGTGAGTGTGT |
| Internal bar code: | CGGACGGAGCCAAAATGTTTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 909 |
| LEAP-Seq percent confirming: | 99.6825 |
| LEAP-Seq n confirming: | 2512 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCCGACAGTGAAGAGTTTG |
| Suggested primer 2: | GAGCACACCTTCTCCTCGTC |