| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.193812 |
| Chromosome: | chromosome 7 |
| Location: | 3785105 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g338350 | CPLD50 | Conserved in the Plant Lineage and Diatoms; (1 of 1) PTHR10072//PTHR10072:SF43 - IRON-SULFUR CLUSTER ASSEMBLY PROTEIN // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGTAGTTTGTAATCCCCCGCCGCCCCCG |
| Internal bar code: | CGAGCGGAGCGGGCACCGCCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 31 |
| LEAP-Seq percent confirming: | 87.5 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTAGTCCACCCTCACCGTA |
| Suggested primer 2: | GCTTCGTGGAGTCCATCTTC |