| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.193814 |
| Chromosome: | chromosome 17 |
| Location: | 2323190 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g714250 | DLC5,DLX2,ODA-LC5 | Flagellar Outer Arm Dynein light chain 5, thioredoxin; (1 of 12) 1.6.5.4 - Monodehydroascorbate reductase (NADH) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCGTCGGGGGTCGGGGGGGGGGCACGAC |
| Internal bar code: | GTGCCGCGGCTGGCGTGGGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 383 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCGGCATCCAGTTCTCTAA |
| Suggested primer 2: | TCACGATCCTGTTGAGTTGC |