Insertion junction: LMJ.RY0402.193816_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.193816
Chromosome:chromosome 5
Location:3189846
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre05.g239700 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCTGCTCGACAAGCAGCTGACGTCATCGTC
Internal bar code:GACAATGGGTGGCGGCCGCAAT

Confirmation - LEAP-Seq

LEAP-Seq distance:641
LEAP-Seq percent confirming:99.6885
LEAP-Seq n confirming:640
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CGTCGCTCCTCCAGTAAGTC
Suggested primer 2:ACGGTAACAAAAGCCACGTC