| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.193827 |
| Chromosome: | chromosome 6 |
| Location: | 5674773 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g286350 | UMM6 | UbiE/COQ5 methyltransferase family protein; (1 of 2) PTHR10108//PTHR10108:SF830 - METHYLTRANSFERASE // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCCTCGCCAGAAAACGGCCGCAGTTTCAG |
| Internal bar code: | GCTGTCACCCCAATGGTCTGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1023 |
| LEAP-Seq percent confirming: | 98.9766 |
| LEAP-Seq n confirming: | 5126 |
| LEAP-Seq n nonconfirming: | 53 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCTATCTCCAATCCGCGAC |
| Suggested primer 2: | TCAAAGTGTCTGTCAAGGCG |