| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.193863 |
| Chromosome: | chromosome_16 |
| Location: | 4952612 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre16.g685450 | DHC8 | Flagellar inner arm dynein heavy chain, dynein e (monomeric) | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TCAAGAAGCTGGGCAACCCGCCCATGATCA |
| Internal bar code: | GCTCCTGAACCGTTGTACGATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 511 |
| LEAP-Seq percent confirming: | 98.659 |
| LEAP-Seq n confirming: | 1030 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACATCAACCCCTACCTGCTG |
| Suggested primer 2: | AAGGCCTCGTTCTTGACGTA |