| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.193874 |
| Chromosome: | chromosome 9 |
| Location: | 2225261 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g392850 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGAGTGTTGCTGCCGGTGGCGATGGAGG |
| Internal bar code: | TATTAGTAATTCAAACATCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 325 |
| LEAP-Seq percent confirming: | 98.6286 |
| LEAP-Seq n confirming: | 4459 |
| LEAP-Seq n nonconfirming: | 62 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAAGCTGTAACGAATGCAA |
| Suggested primer 2: | AGTGCTTTCAGAGCGTGGAT |