| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.193888 |
| Chromosome: | chromosome 3 |
| Location: | 4485781 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g176150 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAGTACTCACTACACGCGGCTTGGACCAT |
| Internal bar code: | GTAATGCGGCCCTGGTCAAGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 603 |
| LEAP-Seq percent confirming: | 98.528 |
| LEAP-Seq n confirming: | 4217 |
| LEAP-Seq n nonconfirming: | 63 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCTGACACACAAATTGCAC |
| Suggested primer 2: | CTTCTTCTTGAGGACCAGCG |