| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.193891 |
| Chromosome: | chromosome 6 |
| Location: | 5016429 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g280227 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATGACCTTATGGCATGGACGGCTTAAAGG |
| Internal bar code: | CGCTGTCAGGTTGATTTTATTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 787 |
| LEAP-Seq percent confirming: | 94.4 |
| LEAP-Seq n confirming: | 118 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCAACAAAAGTGGTACCCG |
| Suggested primer 2: | TCAAGGTGCATCGCTATCTG |