| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.193903 |
| Chromosome: | chromosome_6 |
| Location: | 7503968 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre06.g300500 | CYA18,ADCY1 | Adenylyl cyclase | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GGGGGAGGAGAGACCAGGGGAAGGACAGAC |
| Internal bar code: | TCCGCGTTCAGAAAAGGGTATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 96 |
| LEAP-Seq percent confirming: | 99.8055 |
| LEAP-Seq n confirming: | 513 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGAGTGGAGTGGAGTGGT |
| Suggested primer 2: | GAATCCCTCGTCCTCTTTCC |