| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.193909 |
| Chromosome: | chromosome 1 |
| Location: | 700860 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g003650 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAAGCTTGCTGTGGTGATGTTTCTGAAGC |
| Internal bar code: | TCGGATGAGCTTGGCCATCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 920 |
| LEAP-Seq percent confirming: | 98.7306 |
| LEAP-Seq n confirming: | 700 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCCAGCTTGCTATTAGTGC |
| Suggested primer 2: | AGGAGGGTAGGAAATCCGAA |