| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.193919 |
| Chromosome: | chromosome 1 |
| Location: | 5555136 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g039450 | (1 of 2) PTHR34548//PTHR34548:SF2 - FAMILY NOT NAMED // PROTEIN TIC 21, CHLOROPLASTIC | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACGCGGCATGGTAATGCGGTCGGCTTTCG |
| Internal bar code: | CATTACTCCGGGCCTCCTGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 308 |
| LEAP-Seq percent confirming: | 98.2618 |
| LEAP-Seq n confirming: | 2657 |
| LEAP-Seq n nonconfirming: | 47 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCGAAGAAGGACTTGACTG |
| Suggested primer 2: | TTGGGGAGCACTATTTGGAC |