Insertion junction: LMJ.RY0402.193942_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.193942
Chromosome:chromosome 16
Location:4206753
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre16.g668700 (1 of 37) IPR009030 - Insulin-like growth factor binding protein, N-terminal 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCCATCATGCCCCAACTTATAGTTACATG
Internal bar code:CTGTACAAATTCGCTTCCAGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:407
LEAP-Seq percent confirming:61.194
LEAP-Seq n confirming:205
LEAP-Seq n nonconfirming:130
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GGAGGTTCAGTCGAGCAGTC
Suggested primer 2:CCTCCTACTAGGGCTTCGCT