| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.193950 |
| Chromosome: | chromosome 10 |
| Location: | 2942517 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g440450 | PSB28 | (1 of 1) K08903 - photosystem II 13kDa protein (psb28); Photosystem II associated subunit 28 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGTGGGATCGCTTCATGCGCTTCATGGA |
| Internal bar code: | GGGCGAGAAGGCTAGAGCTCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1077 |
| LEAP-Seq percent confirming: | 99.5434 |
| LEAP-Seq n confirming: | 218 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTACACCCCGCTAACTGACC |
| Suggested primer 2: | TGCGCAGAGGCATACAATAG |