| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.194056 |
| Chromosome: | chromosome 1 |
| Location: | 7269972 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g052500 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAACTACGAGGATAGCTGATGCGTACGCA |
| Internal bar code: | GATAACCGTTAGCGAAAAAACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 504 |
| LEAP-Seq percent confirming: | 99.798 |
| LEAP-Seq n confirming: | 4940 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCTCCTCGCTCTATTGCAT |
| Suggested primer 2: | CACGGACGTTCCCTGATACT |