| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.194072 |
| Chromosome: | chromosome 12 |
| Location: | 4120301 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g518050 | FAP261 | Flagellar Associated Protein 261; (1 of 2) PTHR16306:SF0 - TRANSLIN-ASSOCIATED FACTOR X-INTERACTING PROTEIN 1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGACGGCGGGGCGACCAGGACAGGTGGTG |
| Internal bar code: | GAAGTGTGGGAGGTGCCTAGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 637 |
| LEAP-Seq percent confirming: | 81.6651 |
| LEAP-Seq n confirming: | 2668 |
| LEAP-Seq n nonconfirming: | 599 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGTAGGTAGTGTGTCCCCC |
| Suggested primer 2: | GTATATGGTGGTTAGCGCGG |