| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.194072 |
| Chromosome: | chromosome 13 |
| Location: | 2760630 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g582671 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCCTTGCAACCCCCAGCCCGCCTCCTCCC |
| Internal bar code: | ACGCAGGACGATTTACATCAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 231 |
| LEAP-Seq percent confirming: | 86.4008 |
| LEAP-Seq n confirming: | 4549 |
| LEAP-Seq n nonconfirming: | 716 |
| LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTTGCTCGACTTCGCTCAT |
| Suggested primer 2: | AACACATACTACGAGCCGGG |