| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.194093 |
| Chromosome: | chromosome 3 |
| Location: | 5564592 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g186500 | HAT2 | Histone acetyltransferase 2; (1 of 1) K11303 - histone acetyltransferase 1 (HAT1, KAT1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAGCAACGATGATCGTGACAGGTATGGCA |
| Internal bar code: | CGGATAACACGCGCTAGTTCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 665 |
| LEAP-Seq percent confirming: | 99.7943 |
| LEAP-Seq n confirming: | 3881 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGGGATTCTGAGTGAGTGA |
| Suggested primer 2: | ACAGTGGACAGATGACTGCG |