Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.194150 |
Chromosome: | chromosome_12 |
Location: | 6148013 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre12.g536050 | ALA1 | Phospholipid-transporting ATPase | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TCACAGCCAGGCAGAACCCGTTCCGCCACG |
Internal bar code: | CAGGCGGCTGGAGCAGCCGCGA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 213 |
LEAP-Seq percent confirming: | 99.0826 |
LEAP-Seq n confirming: | 216 |
LEAP-Seq n nonconfirming: | 2 |
LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AACCATGTTTGACAGCACCA |
Suggested primer 2: | CGACAATGCACTCAGCAAGT |