| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.194156 |
| Chromosome: | chromosome_12 |
| Location: | 734845 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre12.g495250 | FAL15 | Similar to Flagellar Associated Protein FAP31 | antisense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTTTCGGCCAGCTCTACTTGCTACACGGAT |
| Internal bar code: | TAGGCTTGGCGCTGGACCACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 410 |
| LEAP-Seq percent confirming: | 99.7516 |
| LEAP-Seq n confirming: | 2008 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCGCATGCAGAGATGTAA |
| Suggested primer 2: | TGTTACTTATGCTGGCACGC |