| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.194189 |
| Chromosome: | chromosome 3 |
| Location: | 628397 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g145727 | (1 of 1) PTHR11946:SF53 - TYROSINE-TRNA LIGASE | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGGTGAGGCTTGGAAATTTTGAGGCATG |
| Internal bar code: | ACCACGTATGTCACGGACCGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 752 |
| LEAP-Seq percent confirming: | 98.4513 |
| LEAP-Seq n confirming: | 445 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTGACCACTTTGAGAACA |
| Suggested primer 2: | TGTGCTGTGGTAAGAAAGCG |