Insertion junction: LMJ.RY0402.194213_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.194213
Chromosome:chromosome 16
Location:7587122
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre16.g689423 (1 of 14) PF00892 - EamA-like transporter family (EamA); Putative UDP-galactose transporter 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AACACCTGCAGACCTGCATGTCGATGCCGC
Internal bar code:GGTCTTTGGTCGCTTAGATGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:368
LEAP-Seq percent confirming:99.7283
LEAP-Seq n confirming:2202
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:ACACACCACCCTGCCTTTAG
Suggested primer 2:AGGTTTGAATGGTGGCAAAG