| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.194228 |
| Chromosome: | chromosome 8 |
| Location: | 2320860 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g370550 | (1 of 1) 1.1.99.39 - D-2-hydroxyglutarate dehydrogenase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCACGCGTCCCGCCCAACCTCCCCACGCA |
| Internal bar code: | GGATTCCGACTTCATTGCCAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1060 |
| LEAP-Seq percent confirming: | 99.6366 |
| LEAP-Seq n confirming: | 5484 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAATGCCGTACGGATCAAC |
| Suggested primer 2: | GAGGAATGGGGATGGAGAAT |