| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.194231 |
| Chromosome: | chromosome 14 |
| Location: | 3548546 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g630950 | (1 of 7) 2.8.1.1 - Thiosulfate sulfurtransferase / Thiosulfate thiotransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCGTGATGTGAGGAGATAGGCAACCCGG |
| Internal bar code: | CATCGCGTCGTTGTCAAGCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1005 |
| LEAP-Seq percent confirming: | 95.3247 |
| LEAP-Seq n confirming: | 1101 |
| LEAP-Seq n nonconfirming: | 54 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATACCCAAGCAAACAGGTGC |
| Suggested primer 2: | CGTCCCTACACGCCTACAAT |