| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.194237 |
| Chromosome: | chromosome 7 |
| Location: | 5599873 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g351850 | NAT21 | (1 of 36) PF00583 - Acetyltransferase (GNAT) family (Acetyltransf_1); N-acetyltransferase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACGTTTCTCATGTCAGAATTTAAGTTCGC |
| Internal bar code: | GCAGCGCGCCGACCTCCGCACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1007 |
| LEAP-Seq percent confirming: | 99.3174 |
| LEAP-Seq n confirming: | 1164 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGGAGGAGCTGTCGATAC |
| Suggested primer 2: | ATGGAATTTGCATGGCTCTC |