| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.194253 |
| Chromosome: | chromosome_2 |
| Location: | 6237507 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g114100 | PDE22 | 3\',5\'-cyclic-nucleotide phosphodiesterase | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTTGAAGCTACTGAATCGTCCATGTATGAT |
| Internal bar code: | TGGGCGTCGGAGCCATCGCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 849 |
| LEAP-Seq percent confirming: | 99.4704 |
| LEAP-Seq n confirming: | 2254 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCCCGCAACTCTAAACTCA |
| Suggested primer 2: | GGATTGGAGTCAGGAGGACA |