Insertion junction: LMJ.RY0402.194280_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.194280
Chromosome:chromosome 9
Location:4525918
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g396028 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCGTACGTAGACCGAGCCAACACGCAGTA
Internal bar code:GCTGTGGCAGTAATCCAATCAA

Confirmation - LEAP-Seq

LEAP-Seq distance:930
LEAP-Seq percent confirming:99.2361
LEAP-Seq n confirming:1299
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:GCTGCCAAACAGGCTACTTC
Suggested primer 2:CCTGCCATAGTCAACACCCT