| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.194321 |
| Chromosome: | chromosome 1 |
| Location: | 6880233 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g049826 | PHC59 | Putative pherophorin-chlamydomonas homolog; (1 of 71) PF12499 - Pherophorin (DUF3707) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTTCATGCTGCGCCGACCGCTGTCGCGC |
| Internal bar code: | TTCTCGGGCGAAGTTCGGGAGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 700 |
| LEAP-Seq percent confirming: | 99.5739 |
| LEAP-Seq n confirming: | 2103 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTCGAATGTACAAGAGCA |
| Suggested primer 2: | TGACAACGCCAACAAATGAT |