| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.194332 |
| Chromosome: | chromosome 1 |
| Location: | 6380493 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g045500 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGGGTAAGTCGAAAATAATGGAACGGGG |
| Internal bar code: | ACTGTGATGATTGTTTGGAATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 601 |
| LEAP-Seq percent confirming: | 98.3819 |
| LEAP-Seq n confirming: | 608 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGGTCGTCTAGTAGCTTCG |
| Suggested primer 2: | GGTCCTAGGGAGAGGGAGTG |