Insertion junction: LMJ.RY0402.194351_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.194351
Chromosome:chromosome 13
Location:3734608
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre13.g589350 FBB6 Flagellar/basal body protein 6; (1 of 1) IPR000679//IPR002035//IPR013694 - Zinc finger, GATA-type // von Willebrand factor, type A // VIT domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGGTGGTGCAGCCCGTGTTGCCTGAGGGC
Internal bar code:ACGACCAGGTCCATGACAGCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:370
LEAP-Seq percent confirming:98.1826
LEAP-Seq n confirming:2269
LEAP-Seq n nonconfirming:42
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GCAGACGGAGTTAGTGCCTC
Suggested primer 2:GAGGTAGCTGAACTCCACCG