Insertion junction: LMJ.RY0402.194372_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.194372
Chromosome:chromosome 4
Location:3863762
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre04.g230340 FAP206 (1 of 1) PTHR21442:SF0 - UPF0704 PROTEIN C6ORF165; Flagellar Associated Protein 206 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGGAGTAGCGCGAATGCCTTCGATGTCGC
Internal bar code:CCGGGATATCGGGCGCGAAACA

Confirmation - LEAP-Seq

LEAP-Seq distance:401
LEAP-Seq percent confirming:96.789
LEAP-Seq n confirming:633
LEAP-Seq n nonconfirming:21
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:TTTGGTTTAGTTTGGGCTGG
Suggested primer 2:TGTTCTTGCTGTTGTAGGCG