| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.194409 |
| Chromosome: | chromosome 9 |
| Location: | 1646091 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g397095 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGGGATGGTTGGCTGGGAAGCTGGGAAC |
| Internal bar code: | CGCCTATGAGCGCCCGTTTGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 781 |
| LEAP-Seq percent confirming: | 99.5765 |
| LEAP-Seq n confirming: | 6819 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTTCTGAAGGAGCTGGTGG |
| Suggested primer 2: | GCAAGGGTATGGAAGGAACA |