| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.194472 |
| Chromosome: | chromosome 9 |
| Location: | 7218416 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g412350 | (1 of 1) PTHR16557:SF4 - 2-OXOGLUTARATE-DEPENDENT DIOXYGENASE FAMILY PROTEIN-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTAGAGAGTCTAGGCGGGCACTCAGCCTG |
| Internal bar code: | ACATGAGCGAAGAGCGACGACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 910 |
| LEAP-Seq percent confirming: | 97.4636 |
| LEAP-Seq n confirming: | 2613 |
| LEAP-Seq n nonconfirming: | 68 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGGTACGTTAGATCGACGG |
| Suggested primer 2: | GCTCGATCACCTGAAGGAAG |