| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.194482 |
| Chromosome: | chromosome 7 |
| Location: | 2721309 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g331150 | (1 of 2) 1.3.3.5 - Bilirubin oxidase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATGTGAACGTCAGCTGCACCAGCGGCAAA |
| Internal bar code: | ATCGGAGACGCAGTGCGGTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 930 |
| LEAP-Seq percent confirming: | 99.6536 |
| LEAP-Seq n confirming: | 2589 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAAGTTGGTGGTCGTGGTA |
| Suggested primer 2: | CTCAACCCCTGATGGAAATG |