| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.194497 |
| Chromosome: | chromosome 9 |
| Location: | 6855115 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g409951 | RuvX homolog; (1 of 1) K07447 - putative holliday junction resolvase (ruvX) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCTTGCTCTTAGCAGGCGCACACGGCCAC |
| Internal bar code: | GCGGCGTGAGGCGTTTTGGGAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 675 |
| LEAP-Seq percent confirming: | 99.689 |
| LEAP-Seq n confirming: | 1282 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCTGTATGCGATTCTGGTT |
| Suggested primer 2: | GGCTGCTTTCACCTCTCATC |